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Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 329-333 doi: 10.1007/s11783-007-0056-7

Abstract: In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established andg/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouseThe assay showed high relativity with high performance liquid chromatography (HPLC) with a correlation

Keywords： o-phenylenediamine     4-arginine     ic-ELISA     substrate     chromatography    

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Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 3,   Pages 381-384 doi: 10.1007/s11783-007-0065-6

Abstract: This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA

Keywords： conventional     sandwich     pretreatment     enzyme-linked immunosorbent     detection    

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Preparation of hapten-specific monoclonal antibody for cadmium and its ELISA application to aqueous samples

Huan HE, Bo TANG, Cheng SUN, Shaogui YANG, Weijuan ZHENG, Zichun HUA

Frontiers of Environmental Science & Engineering 2011, Volume 5, Issue 3,   Pages 409-416 doi: 10.1007/s11783-011-0349-8

Abstract: Competitive enzyme-linked immunosorbent assay (ELISA) for quantitative detection of cadmium in aqueousThe intra- and inter-assay coefficient variations were 1.5%–6.3% and 3.2%–7.4%, respectively.The detection limit of this assay was well below the allowable concentration of cadmium (3 μg·L ), and

Keywords： cadmium     hapten     monoclonal antibody     enzyme-linked immunosorbent assay (ELISA)    

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Analysis of 2 046 cases of TORCH infection in women before pregnancy in Guangzhou

Yu Zhiheng

Strategic Study of CAE 2014, Volume 16, Issue 5,   Pages 63-66

Abstract:

To investigate the infective rates of toxoplasma，Rubella virus，cytomegalovirus，herpes simplex virus (TORCH ) in unborn child and pregnant women in Guangzhou City and offer the reference for maternity and child care．We detected the TORCH IgM and IgG from Jan 2013 to December 2013，serum samples from 2 046 women before pregnancy and in the early period of pregnancy in our research institute were tested by ELISA. In the survey of 2 046 women，the total positive rate of TORCH-IgM was 0.88 %，with the higher positive Rates of CMV (0.68 %) and TOX(0.20 %). In the positive Rates of TORCH-IgG，CMV was the highest，up to 97.65 %，followed by RV (85.58 %) and TOXO (4.89 %)．The infective rate of TORCH is pretty high in women before pregnancy and in the early period of pregnancy in Guangzhou，monitor actively and strengthen publicity of TORCH，early detection and early intervention，improving the quality of premarital births and reducing the occurrence of birth defects and disabilities．

Keywords： TORCH     infective rate     enzyme- linked immunosorbent assay     birth- defect     pregnant examination    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , Yongguo HUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Frontiers of Medicine 2009, Volume 3, Issue 3,   Pages 277-283 doi: 10.1007/s11684-009-0047-0

Abstract: Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), aThe assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-typeThe results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125&

Keywords： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbentassay    

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Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines

Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN

Frontiers of Medicine 2009, Volume 3, Issue 2,   Pages 148-152 doi: 10.1007/s11684-009-0032-7

Abstract: amplified by polymerase chain reaction (PCR) and cloned into pGL3-basic vector and pEGFP-1 vector to assayThe promoter transcription activity was determined by dual-luciferase report assay, and enhanced greenThe dual-luciferase reporter assay showed that the promoter transcription activity in digestive system

Keywords： gene     X-linked inhibitor of apoptosis protein associated factor-1 (XAF1)     promoter     transcription regulation    

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Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 139-142 doi: 10.1007/s11705-017-1636-0

Abstract: In this commentary, discovery story of a “lactate-polymerizing enzyme” (LPE)?and LPE-based?

Keywords： synthetic biology     enzyme evolutionary engineering     polyhydroxyalkanoate    

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Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Frontiers of Environmental Science & Engineering 2007, Volume 1, Issue 1,   Pages 89-94 doi: 10.1007/s11783-007-0017-1

Abstract: The present work studied the influence of glucose feeding on the ligninolytic enzyme production of inthe first day of the culture (24 h after the inoculation) stimulated both fungal biomass growth and enzymeof MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme

Keywords： enzyme production     fed-batch culture     nitrogen-limited     inoculation     mmol/L    

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Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 5, doi: 10.1007/s11783-022-1567-y

Abstract:

● Evaluated three methods for determining the consortia’s growth kinetics.

Keywords： Biodegradation     1     4-Dioxane     Kinetics     Microbial consortium     16S rRNA    

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Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Frontiers of Medicine 2022, Volume 16, Issue 2,   Pages 157-175 doi: 10.1007/s11684-021-0884-z

Abstract: In this review, the latest advances of biosensors regarding the assay of exosomes were summarized, and

Keywords： biosensor     exosome     cancer diagnosis    

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Diffusion process in enzyme–metal hybrid catalysts

Frontiers of Chemical Science and Engineering 2022, Volume 16, Issue 6,   Pages 921-929 doi: 10.1007/s11705-022-2144-4

Abstract: Enzyme–metal hybrid catalysts bridge the gap between enzymatic and heterogeneous catalysis, which isPrevious studies have demonstrated that the enzyme–metal hybrid catalysts exhibited considerably highercatalytic efficiency in cascade reactions, compared with that of the combination of separated enzymeHere, we investigated the diffusion process in enzyme–metal hybrid catalysts using Pd/lipase-Pluronicand metal active sites, which provides a theoretical foundation for the rational design of enzyme–metal

Keywords： enzyme–metal hybrid catalyst     internal diffusion     proximity effect     kinetic model    

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Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 6,   Pages 784-794 doi: 10.1007/s11705-022-2278-4

Abstract: In situ encapsulation is an effective way to synthesize enzyme@metal–organic framework biocatalysts

Keywords： bismuth-ellagic acid     in situ encapsulation     enzyme@MOF biocomposites    

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Soil enzyme activities and their indication for fertility of urban forest soil

SHAN Qihua, YU Yuanchun, ZHANG Jinchi, YU Jian

Frontiers of Environmental Science & Engineering 2008, Volume 2, Issue 2,   Pages 218-223 doi: 10.1007/s11783-008-0037-5

Abstract: To reveal the biological characteristics of urban forest soil and the effects of soil enzyme on soilfertility as well as the correlation between physicochemical properties and enzyme activities, 44 urbanBasic soil physicochemical properties and enzyme activities were analyzed in the laboratory.Both in 0–20 cm and 20–40 cm soil, soil enzyme system (hydrogen peroxidase, dehydrogenase, alkaline phosphataseThe more soil enzyme activities there were, the higher the fertility of urban forest soil.
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Room temperature preparation of hydrazine-linked covalent organic frameworks coated capillaries for

Frontiers of Chemical Science and Engineering 2023, Volume 17, Issue 5,   Pages 548-556 doi: 10.1007/s11705-022-2252-1

Abstract: In this work, hydrazine-linked TFPB-DHzDS (TFPB: 1,3,5-tris(4-formylphenyl)benzene; DHzDS: 2,5-bis(3-

Keywords： hydrazine-linked TFPB-DHzDS     in-situ growth method     open-tubular capillary electrochromatography    

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Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 1,   Pages 66-71 doi: 10.1007/s11705-016-1591-1

Abstract: insufficient supply of NADH, tends to limit their production yields in case of utilizing NADH-dependent enzyme

Keywords： NADH-dependent enzyme     NADPH-dependent enzyme     transhydrogenase     site-directed mutagenesis     enzyme engineering    

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Title Author Date Type Operation

Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

Journal Article

Detection of Escherichia coli in wastewater based on enzyme immunoassay

XI Haiyan, CAI Qiang, HE Miao, SHI Hanchang

Journal Article

Preparation of hapten-specific monoclonal antibody for cadmium and its ELISA application to aqueous samples

Huan HE, Bo TANG, Cheng SUN, Shaogui YANG, Weijuan ZHENG, Zichun HUA

Journal Article

Analysis of 2 046 cases of TORCH infection in women before pregnancy in Guangzhou

Yu Zhiheng

Journal Article

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , Yongguo HUANG MS , Jingsong GONG BM , Yan CHEN BM ,

Journal Article

Cloning of human XAF1 gene promoter and assay of its transcription activity in a variety of cell lines

Qiong CHEN, Qing YU, Yuhu SONG, Peiyuan Li, Ying CHANG, Zhijun WANG, Lifeng LIU, Wei WU, Jusheng LIN

Journal Article

Designer enzyme for green materials innovation: Lactate-polymerizing enzyme as a key catalyst

Seiichi Taguchi

Journal Article

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete

ZHOU Xiaoyan, WEN Xianghua, FENG Yan

Journal Article

Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

Journal Article

Biosensor-based assay of exosome biomarker for early diagnosis of cancer

Journal Article

Diffusion process in enzyme–metal hybrid catalysts

Journal Article

Enzyme@bismuth-ellagic acid: a versatile platform for enzyme immobilization with enhanced acid-base stability

Journal Article

Soil enzyme activities and their indication for fertility of urban forest soil

SHAN Qihua, YU Yuanchun, ZHANG Jinchi, YU Jian

Journal Article

Room temperature preparation of hydrazine-linked covalent organic frameworks coated capillaries for

Journal Article

Cofactor engineering in cyanobacteria to overcome imbalance between NADPH and NADH: A mini review

Jongmoon Park,Yunnam Choi

Journal Article